Mouse model of Schistosomiasis: infection with Schistosoma mansoni in CD-1 mice

Schistosomiasis is a parasitic disease that affects almost 240 million worldwide. CD1 mice were infected with cercariae of S. mansoni, after which infection developed for 8 weeks. Tissues were processed to immuno-histological techniques. It was performed H&E staining for overall analyses, Sirius Red for fibrosis and immunohistochemistry for inflammation biomarkers. The most infected organ was the liver, fibrosis decreased with egg development and Galectin-3 (Gal3) and Interleukin 6 (IL-6) were expressed inside granulomasThis work was also supported by FCT – Fundação para a Ciência e Tecnologia (REF UID/BIM/04293/2013) and by the project NORTE-01-0145-FEDER-000012 and by a scholarship to Carla Luís with the reference SAICT2016/FEDER/BIO4DIA/BTI under the supervision of Dr. Rúben Fernandes.N/

Solar type II radio bursts associated with CME expansions as shown by EUV waves

We investigate the physical conditions of the sources of two metric Type-II bursts associated with CME expansions with the aim of verifying the relationship between the shocks and the CMEs, comparing the heights of the radio sources and the heights of the EUV waves associated with the CMEs. The heights of the EUV waves associated with the events were determined in relation to the wave fronts. The heights of the shocks were estimated by applying two different density models to the frequencies of the Type-II emissions and compared with the heights of the EUV waves. For the 13 June 2010 event, with band-splitting, the shock speed was estimated from the frequency drifts of the upper and lower branches of the harmonic lane, taking into account the H/F frequency ratio fH/fF = 2. Exponential fits on the intensity maxima of the branches revealed to be more consistent with the morphology of the spectrum of this event. For the 6 June 2012 event, with no band-splitting and with a clear fundamental lane on the spectrum, the shock speed was estimated directly from the frequency drift of the fundamental emission, determined by linear fit on the intensity maxima of the lane. For each event, the most appropriate density model was adopted to estimate the physical parameters of the radio source. The 13 June 2010 event presented a shock speed of 664-719 km/s, consistent with the average speed of the EUV wave fronts of 609 km/s. The 6 June 2012 event was related to a shock of speed of 211-461 km/s, also consistent with the average speed of the EUV wave fronts of 418 km/s. For both events, the heights of the EUV wave revealed to be compatible with the heights of the radio source, assuming a radial propagation of the shock.Comment: Accepted for publication in Astronomy and Astrophysic

Superlens made of a metamaterial with extreme effective parameters

We propose a superlens formed by an ultra-dense array of crossed metallic wires. It is demonstrated that due to the anomalous interaction between crossed wires, the structured substrate is characterized by an anomalously high index of refraction and supports strongly confined guided modes with very short propagation wavelengths. It is theoretically proven that a planar slab of such structured material makes a superlens that may compensate for the attenuation introduced by free-space propagation and restore the subwavelength details of the source. The bandwidth of the proposed device can be quite significant since the response of the structured substrate is non-resonant. The theoretical results are fully supported by numerical simulations.Comment: Accepted for publication in Phys. Rev. B (in press

BOND: Bayesian Oxygen and Nitrogen abundance Determinations in giant H II regions using strong and semi-strong lines

We present BOND, a Bayesian code to simultaneously derive oxygen and nitrogen abundances in giant H II regions. It compares observed emission lines to a grid of photoionization models without assuming any relation between O/H and N/O. Our grid spans a wide range in O/H, N/O and ionization parameter U, and covers different starburst ages and nebular geometries. Varying starburst ages accounts for variations in the ionizing radiation field hardness, which arise due to the ageing of H II regions or the stochastic sampling of the initial mass function. All previous approaches assume a strict relation between the ionizing field and metallicity. The other novelty is extracting information on the nebular physics from semi-strong emission lines. While strong lines ratios alone ([O III]/Hbeta, [O II]/Hbeta and [N II]/Hbeta) lead to multiple O/H solutions, the simultaneous use of [Ar III]/[Ne III] allows one to decide whether an H II region is of high or low metallicity. Adding He I/Hbeta pins down the hardness of the radiation field. We apply our method to H II regions and blue compact dwarf galaxies, and find that the resulting N/O vs O/H relation is as scattered as the one obtained from the temperature-based method. As in previous strong-line methods calibrated on photoionization models, the BOND O/H values are generally higher than temperature-based ones, which might indicate the presence of temperature fluctuations or kappa distributions in real nebulae, or a too soft ionizing radiation field in the models.Comment: MNRAS in press; 21 pages, 22 figures, 2 tables; code, data and results available at http://bond.ufsc.b

Transition (LINER/HII) nuclei as evolved Composite (Seyfert 2/Starburst) nuclei

We compare the circumnuclear stellar population and environmental properies of Seyfert and Composite (Seyfert + Starburst) nuclei with those of LINERs and LINER/HII transition galaxies (TOs), and discuss evidences for evolution from Seyfert/Composite to LINER/TO nuclei.Comment: 2 pages, 1 figure; to appear in the Proceedings of IAU Symp. No. 222: The Interplay among Black Holes, Stars and ISM in Galactic Nuclei, CUP, eds. T. Storchi-Bergmann, L. Ho and H. R. Schmit

Assessing individual differences in genome-wide gene expression in human whole blood: reliability over four hours and stability over 10 months.

Studying the causes and correlates of natural variation in gene expression in healthy populations assumes that individual differences in gene expression can be reliably and stably assessed across time. However, this is yet to be established. We examined 4-hour test-retest reliability and 10 month test-retest stability of individual differences in gene expression in ten 12-year-old children. Blood was collected on four occasions: 10 a.m. and 2 p.m. on Day 1 and 10 months later at 10 a.m. and 2 p.m. Total RNA was hybridized to Affymetrix-U133 plus 2.0 arrays. For each probeset, the correlation across individuals between 10 a.m. and 2 p.m. on Day 1 estimates test-retest reliability. We identified 3,414 variable and abundantly expressed probesets whose 4-hour test-retest reliability exceeded .70, a conventionally accepted level of reliability, which we had 80% power to detect. Of the 3,414 reliable probesets, 1,752 were also significantly reliable 10 months later. We assessed the long-term stability of individual differences in gene expression by correlating the average expression level for each probe-set across the two 4-hour assessments on Day 1 with the average level of each probe-set across the two 4-hour assessments 10 months later. 1,291 (73.7%) of the 1,752 probe-sets that reliably detected individual differences across 4 hours on two occasions, 10 months apart, also stably detected individual differences across 10 months. Heritability, as estimated from the MZ twin intraclass correlations, is twice as high for the 1,752 reliable probesets versus all present probesets on the array (0.68 vs 0.34), and is even higher (0.76) for the 1,291 reliable probesets that are also stable across 10 months. The 1,291 probesets that reliably detect individual differences from a single peripheral blood collection and stably detect individual differences over 10 months are promising targets for research on the causes (e.g., eQTLs) and correlates (e.g., psychopathology) of individual differences in gene expression